Article: article from journal or magazin.
Investigation of the hepatitis C virus replication complex.
Methods in Molecular Biology
Formation of a membrane-associated replication complex, composed of viral proteins, replicating RNA, altered cellular membranes, and other host factors, is a hallmark of all positive-strand RNA viruses. In the case of HCV, RNA replication takes place in a likely endoplasmic reticulum-derived membrane alteration referred to as the "membranous web." In vitro transcription-translation, membrane extraction and flotation analyses, immunofluorescence microscopy, fluorescent in situ hybridization, and RNA metabolic labeling followed by confocal laser scanning microscopy have yielded insights into the structure and function of the HCV replication complex. We describe these techniques and highlight selected results.
Animals, Bromodeoxyuridine/metabolism, Cell Membrane/metabolism, Fluorescent Antibody Technique, Hepacivirus/metabolism, In Situ Hybridization, Fluorescence, Protein Biosynthesis, RNA, Viral/biosynthesis, Staining and Labeling, Transcription, Genetic, Viral Proteins/biosynthesis, Viral Proteins/chemistry, Virus Replication
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