SMNrp is an essential pre-mRNA splicing factor required for the formation of the mature spliceosome.

Details

Serval ID
serval:BIB_19632
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
SMNrp is an essential pre-mRNA splicing factor required for the formation of the mature spliceosome.
Journal
EMBO Journal
Author(s)
Meister G., Hannus S., Plöttner O., Baars T., Hartmann E., Fakan S., Laggerbauer B., Fischer U.
ISSN
0261-4189
Publication state
Published
Issued date
2001
Volume
20
Number
9
Pages
2304-2314
Language
english
Notes
Publication types: Journal Article
Abstract
SMNrp, also termed SPF30, has recently been identified in spliceosomes assembled in vitro. We have functionally characterized this protein and show that it is an essential splicing factor. We show that SMNrp is a 17S U2 snRNP-associated protein that appears in the pre-spliceosome (complex A) and the mature spliceosome (complex B) during splicing. Immunodepletion of SMNrp from nuclear extract inhibits the first step of pre-mRNA splicing by preventing the formation of complex B. Re-addition of recombinant SMNrp to immunodepleted extract reconstitutes both spliceosome formation and splicing. Mutations in two domains of SMNrp, although similarly deleterious for splicing, differed in their consequences on U2 snRNP binding, suggesting that SMNrp may also engage in interactions with splicing factors other than the U2 snRNP. In agreement with this, we present evidence for an additional interaction between SMNrp and the [U4/U6.U5] tri-snRNP. A candidate that may mediate this interaction, namely the U4/U6-90 kDa protein, has been identified. We suggest that SMNrp, as a U2 snRNP-associated protein, facilitates the recruitment of the [U4/U6.U5] tri-snRNP to the pre-spliceosome.
Keywords
Animals, Cell Line, Cell Nucleus/metabolism, Cyclic AMP Response Element-Binding Protein, Humans, Mutation, Nerve Tissue Proteins/genetics, Nerve Tissue Proteins/metabolism, Protein Binding/genetics, Protein Structure, Tertiary/physiology, RNA Precursors/metabolism, RNA Splicing/physiology, RNA-Binding Proteins/metabolism, Recombinant Proteins/genetics, Recombinant Proteins/metabolism, Ribonucleoprotein, U2 Small Nuclear/metabolism, Ribonucleoprotein, U4-U6 Small Nuclear/metabolism, Ribonucleoproteins, Small Nuclear/metabolism, SMN Complex Proteins, Spliceosomes/metabolism, Transcription, Genetic, Xenopus
Pubmed
Web of science
Open Access
Yes
Create date
19/11/2007 12:14
Last modification date
20/08/2019 12:50
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