SMNrp is an essential pre-mRNA splicing factor required for the formation of the mature spliceosome.

Détails

ID Serval
serval:BIB_19632
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
SMNrp is an essential pre-mRNA splicing factor required for the formation of the mature spliceosome.
Périodique
EMBO Journal
Auteur(s)
Meister G., Hannus S., Plöttner O., Baars T., Hartmann E., Fakan S., Laggerbauer B., Fischer U.
ISSN
0261-4189
Statut éditorial
Publié
Date de publication
2001
Volume
20
Numéro
9
Pages
2304-2314
Langue
anglais
Notes
Publication types: Journal Article
Résumé
SMNrp, also termed SPF30, has recently been identified in spliceosomes assembled in vitro. We have functionally characterized this protein and show that it is an essential splicing factor. We show that SMNrp is a 17S U2 snRNP-associated protein that appears in the pre-spliceosome (complex A) and the mature spliceosome (complex B) during splicing. Immunodepletion of SMNrp from nuclear extract inhibits the first step of pre-mRNA splicing by preventing the formation of complex B. Re-addition of recombinant SMNrp to immunodepleted extract reconstitutes both spliceosome formation and splicing. Mutations in two domains of SMNrp, although similarly deleterious for splicing, differed in their consequences on U2 snRNP binding, suggesting that SMNrp may also engage in interactions with splicing factors other than the U2 snRNP. In agreement with this, we present evidence for an additional interaction between SMNrp and the [U4/U6.U5] tri-snRNP. A candidate that may mediate this interaction, namely the U4/U6-90 kDa protein, has been identified. We suggest that SMNrp, as a U2 snRNP-associated protein, facilitates the recruitment of the [U4/U6.U5] tri-snRNP to the pre-spliceosome.
Mots-clé
Animals, Cell Line, Cell Nucleus/metabolism, Cyclic AMP Response Element-Binding Protein, Humans, Mutation, Nerve Tissue Proteins/genetics, Nerve Tissue Proteins/metabolism, Protein Binding/genetics, Protein Structure, Tertiary/physiology, RNA Precursors/metabolism, RNA Splicing/physiology, RNA-Binding Proteins/metabolism, Recombinant Proteins/genetics, Recombinant Proteins/metabolism, Ribonucleoprotein, U2 Small Nuclear/metabolism, Ribonucleoprotein, U4-U6 Small Nuclear/metabolism, Ribonucleoproteins, Small Nuclear/metabolism, SMN Complex Proteins, Spliceosomes/metabolism, Transcription, Genetic, Xenopus
Pubmed
Web of science
Open Access
Oui
Création de la notice
19/11/2007 13:14
Dernière modification de la notice
20/08/2019 13:50
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