In vivo neurochemical measurements in cerebral tissues using a droplet-based monitoring system.

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Version: Final published version
Serval ID
serval:BIB_1793CE3D7607
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
In vivo neurochemical measurements in cerebral tissues using a droplet-based monitoring system.
Journal
Nature communications
Author(s)
Petit-Pierre G., Colin P., Laurer E., Déglon J., Bertsch A., Thomas A., Schneider B.L., Renaud P.
ISSN
2041-1723 (Electronic)
ISSN-L
2041-1723
Publication state
Published
Issued date
01/11/2017
Peer-reviewed
Oui
Volume
8
Number
1
Pages
1239
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Direct collection of extracellular fluid (ECF) plays a central role in the monitoring of neurological disorders. Current approaches using microdialysis catheters are however drastically limited in term of temporal resolution. Here we show a functional in vivo validation of a droplet collection system included at the tip of a neural probe. The system comprises an advanced droplet formation mechanism which enables the collection of neurochemicals present in the brain ECF at high-temporal resolution. The probe was implanted in a rat brain and could successfully collect fluid samples organized in a train of droplets. A microfabricated target plate compatible with most of the surface-based detection methods was specifically developed for sample analysis. The time-resolved brain-fluid samples are analyzed using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The results provide a time evolution picture of the cerebral tissues neurochemical composition for selected elements known for their involvement in neurodegenerative diseases.
Keywords
Animals, Brain, Brain Chemistry, Calcium/analysis, Cerebrum/chemistry, Extracellular Fluid/chemistry, Female, Magnesium/analysis, Mass Spectrometry, Mercury/analysis, Microdialysis/methods, Potassium/analysis, Rats, Rats, Sprague-Dawley, Sodium/analysis, Time Factors
Pubmed
Web of science
Open Access
Yes
Create date
03/11/2017 12:17
Last modification date
20/08/2019 12:47
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