Strain-specific humoral response to a polymorphic malaria vaccine
Details
Serval ID
serval:BIB_17318F3041E1
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Strain-specific humoral response to a polymorphic malaria vaccine
Journal
Infection and Immunity
ISSN
0019-9567 (Print)
Publication state
Published
Issued date
11/2004
Volume
72
Number
11
Pages
6300-5
Notes
Clinical Trial
Clinical Trial, Phase I
Clinical Trial, Phase II
Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't --- Old month value: Nov
Clinical Trial, Phase I
Clinical Trial, Phase II
Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't --- Old month value: Nov
Abstract
The 3D7 form of the merozoite surface protein 2 (MSP2) of Plasmodium falciparum was one of three subunits of the malaria vaccine Combination B that were tested in a phase I/IIb double-blind randomized placebo-controlled trial, which was undertaken with 120 Papua New Guinean children of 5 to 9 years of age. Because only one variant of the highly polymorphic MSP2 was used for vaccination, we examined whether the elicited response was directed against conserved or strain-specific epitopes. Postvaccination (week 12) titers of antibody against recombinantly expressed individual domains of MSP2 were measured by enzyme-linked immunosorbent assay and compared to baseline values. We found that vaccination with the 3D7 form of MSP2 induced a significant strain-specific humoral response directed against the repetitive and semiconserved family-specific part. The conserved N- and C-terminal domains were not immunogenic. Titers of antibody against the alternate FC27 family-specific domain showed a tendency to increase in vaccinated children, but there was no increase in antibodies against FC27-type 32-mer repeats. These results indicate that vaccination with one MSP2 variant mainly induced a strain-specific response, which can explain the selective effect of vaccination with combination B on the genotypes of breakthrough parasites. These findings support the inclusion of both family-specific domains (3D7 and FC27) in an improved vaccine formulation.
Keywords
Amino Acid Sequence
Animals
Antibodies, Protozoan/*blood
Antigens, Protozoan/chemistry/genetics/*immunology
Child
Child, Preschool
Double-Blind Method
Humans
Malaria Vaccines/administration & dosage/*immunology
Malaria, Falciparum/*immunology/parasitology/prevention & control
New Guinea
Peptides/chemical synthesis/chemistry/genetics/immunology
Plasmodium falciparum/classification/genetics/*immunology
*Polymorphism, Genetic
Protozoan Proteins/chemistry/genetics/*immunology
Recombinant Proteins/immunology
Species Specificity
Vaccination
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 11:49
Last modification date
20/08/2019 12:46