Strain-specific humoral response to a polymorphic malaria vaccine

Détails

ID Serval
serval:BIB_17318F3041E1
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Strain-specific humoral response to a polymorphic malaria vaccine
Périodique
Infection and Immunity
Auteur(s)
Fluck  C., Smith  T., Beck  H. P., Irion  A., Betuela  I., Alpers  M. P., Anders  R., Saul  A., Genton  B., Felger  I.
ISSN
0019-9567 (Print)
Statut éditorial
Publié
Date de publication
11/2004
Volume
72
Numéro
11
Pages
6300-5
Notes
Clinical Trial
Clinical Trial, Phase I
Clinical Trial, Phase II
Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't --- Old month value: Nov
Résumé
The 3D7 form of the merozoite surface protein 2 (MSP2) of Plasmodium falciparum was one of three subunits of the malaria vaccine Combination B that were tested in a phase I/IIb double-blind randomized placebo-controlled trial, which was undertaken with 120 Papua New Guinean children of 5 to 9 years of age. Because only one variant of the highly polymorphic MSP2 was used for vaccination, we examined whether the elicited response was directed against conserved or strain-specific epitopes. Postvaccination (week 12) titers of antibody against recombinantly expressed individual domains of MSP2 were measured by enzyme-linked immunosorbent assay and compared to baseline values. We found that vaccination with the 3D7 form of MSP2 induced a significant strain-specific humoral response directed against the repetitive and semiconserved family-specific part. The conserved N- and C-terminal domains were not immunogenic. Titers of antibody against the alternate FC27 family-specific domain showed a tendency to increase in vaccinated children, but there was no increase in antibodies against FC27-type 32-mer repeats. These results indicate that vaccination with one MSP2 variant mainly induced a strain-specific response, which can explain the selective effect of vaccination with combination B on the genotypes of breakthrough parasites. These findings support the inclusion of both family-specific domains (3D7 and FC27) in an improved vaccine formulation.
Mots-clé
Amino Acid Sequence Animals Antibodies, Protozoan/*blood Antigens, Protozoan/chemistry/genetics/*immunology Child Child, Preschool Double-Blind Method Humans Malaria Vaccines/administration & dosage/*immunology Malaria, Falciparum/*immunology/parasitology/prevention & control New Guinea Peptides/chemical synthesis/chemistry/genetics/immunology Plasmodium falciparum/classification/genetics/*immunology *Polymorphism, Genetic Protozoan Proteins/chemistry/genetics/*immunology Recombinant Proteins/immunology Species Specificity Vaccination
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/01/2008 11:49
Dernière modification de la notice
20/08/2019 12:46
Données d'usage