Pushing Raman spectroscopy over the edge: purported signatures of organic molecules in fossil animals are instrumental artefacts

Details

Ressource 1Download: Alleon-etal-preprint.pdf (3153.55 [Ko])
State: Public
Version: author
License: CC BY 4.0
Serval ID
serval:BIB_147F8BFA5DAE
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Pushing Raman spectroscopy over the edge: purported signatures of organic molecules in fossil animals are instrumental artefacts
Journal
BioEssays
Author(s)
Alleon Julien, Montagnac Gilles, Reynard Bruno, Brulé Thibault, Thoury Mathieu, Gueriau Pierre
ISSN
0265-9247
ISSN-L
1521-1878
Publication state
Published
Issued date
04/2021
Peer-reviewed
Oui
Volume
43
Number
4
Pages
2000295
Language
english
Abstract
Widespread preservation of fossilized biomolecules in many fossil animals has recently been reported in six studies, based on Raman microspectroscopy. Here, we show that the putative Raman signatures of organic compounds in these fossils are actually instrumental artefacts resulting from intense background luminescence. Raman spectroscopy is based on the detection of photons scattered inelastically by matter upon its interaction with a laser beam. For many natural materials, this interaction also generates a luminescence signal that is often orders of magnitude more intense than the light produced by Raman scattering. Such luminescence, coupled with the transmission properties of the spectrometer, induced quasi-periodic ripples in the measured spectra that have been incorrectly interpreted as Raman signatures of organic molecules. Although several analytical strategies have been developed to overcome this common issue, Raman microspectroscopy as used in the studies questioned here cannot be used to identify fossil biomolecules.
Keywords
Raman microspectrosopy, Palaeontology, fossils, luminescence, signal processing
Research datasets
Funding(s)
Swiss National Science Foundation / Projects
Create date
19/01/2021 21:27
Last modification date
25/02/2023 6:46
Usage data