The Genome of the Toluene-Degrading Pseudomonas veronii Strain 1YdBTEX2 and Its Differential Gene Expression in Contaminated Sand.

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State: Public
Version: author
Serval ID
serval:BIB_14197457242A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The Genome of the Toluene-Degrading Pseudomonas veronii Strain 1YdBTEX2 and Its Differential Gene Expression in Contaminated Sand.
Journal
PloS one
Author(s)
Morales M., Sentchilo V., Bertelli C., Komljenovic A., Kryuchkova-Mostacci N., Bourdilloud A., Linke B., Goesmann A., Harshman K., Segers F., Delapierre F., Fiorucci D., Seppey M., Trofimenco E., Berra P., El Taher A., Loiseau C., Roggero D., Sulfiotti M., Etienne A., Ruiz Buendia G., Pillard L., Escoriza A., Moritz R., Schneider C., Alfonso E., Ben Jeddou F., Selmoni O., Resch G., Greub G., Emery O., Dubey M., Pillonel T., Robinson-Rechavi M., van der Meer J.R.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Publication state
Published
Issued date
2016
Peer-reviewed
Oui
Volume
11
Number
11
Pages
e0165850
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish

Abstract
The natural restoration of soils polluted by aromatic hydrocarbons such as benzene, toluene, ethylbenzene and m- and p-xylene (BTEX) may be accelerated by inoculation of specific biodegraders (bioaugmentation). Bioaugmentation mainly involves introducing bacteria that deploy their metabolic properties and adaptation potential to survive and propagate in the contaminated environment by degrading the pollutant. In order to better understand the adaptive response of cells during a transition to contaminated material, we analyzed here the genome and short-term (1 h) changes in genome-wide gene expression of the BTEX-degrading bacterium Pseudomonas veronii 1YdBTEX2 in non-sterile soil and liquid medium, both in presence or absence of toluene. We obtained a gapless genome sequence of P. veronii 1YdBTEX2 covering three individual replicons with a total size of 8 Mb, two of which are largely unrelated to current known bacterial replicons. One-hour exposure to toluene, both in soil and liquid, triggered massive transcription (up to 208-fold induction) of multiple gene clusters, such as toluene degradation pathway(s), chemotaxis and toluene efflux pumps. This clearly underlines their key role in the adaptive response to toluene. In comparison to liquid medium, cells in soil drastically changed expression of genes involved in membrane functioning (e.g., lipid composition, lipid metabolism, cell fatty acid synthesis), osmotic stress response (e.g., polyamine or trehalose synthesis, uptake of potassium) and putrescine metabolism, highlighting the immediate response mechanisms of P. veronii 1YdBTEX2 for successful establishment in polluted soil.

Keywords
Dehydration, Gene Expression Profiling, Microarray Analysis, Micrococcaceae/genetics, Micrococcaceae/metabolism, Pseudomonas/genetics, Pseudomonas/growth & development, Pseudomonas/metabolism, Sphingomonas/genetics, Sphingomonas/metabolism, Water Pollutants/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
28/11/2016 11:27
Last modification date
17/06/2020 14:48
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