Reduced peroxisomal citrate synthase activity increases substrate availability for polyhydroxyalkanoate biosynthesis in plant peroxisomes.

Details

Serval ID
serval:BIB_137DC9FB61B0
Type
Article: article from journal or magazin.
Collection
Publications
Title
Reduced peroxisomal citrate synthase activity increases substrate availability for polyhydroxyalkanoate biosynthesis in plant peroxisomes.
Journal
Plant Biotechnology Journal
Author(s)
Tilbrook K., Poirier Y., Gebbie L., Schenk P.M., McQualter R.B., Brumbley S.M.
ISSN
1467-7652 (Electronic)
ISSN-L
1467-7644
Publication state
Published
Issued date
2014
Peer-reviewed
Oui
Volume
12
Number
8
Pages
1044-1052
Language
english
Abstract
Polyhydroxyalkanoates (PHAs) are bacterial carbon storage polymers used as renewable, biodegradable plastics. PHA production in plants may be a way to reduce industrial PHA production costs. We recently demonstrated a promising level of peroxisomal PHA production in the high biomass crop species sugarcane. However, further production strategies are needed to boost PHA accumulation closer to commercial targets. Through exogenous fatty acid feeding of Arabidopsis thaliana plants that contain peroxisome-targeted PhaA, PhaB and PhaC enzymes from Cupriavidus necator, we show here that the availability of substrates derived from the β-oxidation cycle limits peroxisomal polyhydroxybutyrate (PHB) biosynthesis. Knockdown of peroxisomal citrate synthase activity using artificial microRNA increased PHB production levels approximately threefold. This work demonstrates that reduction of peroxisomal citrate synthase activity may be a valid metabolic engineering strategy for increasing PHA production in other plant species.
Keywords
polyhydroxyalkanoate, citrate synthase, beta-oxidation, peroxisome, plant metabolic engineering, Arabidopsis
Pubmed
Web of science
Open Access
Yes
Create date
28/10/2014 12:50
Last modification date
20/08/2019 13:42
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