Are TADs supercoiled?

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Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_12BCCBEC8B1A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Are TADs supercoiled?
Journal
Nucleic acids research
Author(s)
Racko D., Benedetti F., Dorier J., Stasiak A.
ISSN
1362-4962 (Electronic)
ISSN-L
0305-1048
Publication state
Published
Issued date
25/01/2019
Peer-reviewed
Oui
Volume
47
Number
2
Pages
521-532
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Review
Publication Status: ppublish
Abstract
Topologically associating domains (TADs) are megabase-sized building blocks of interphase chromosomes in higher eukaryotes. TADs are chromosomal regions with increased frequency of internal interactions. On average a pair of loci separated by a given genomic distance contact each other 2-3 times more frequently when they are in the same TAD as compared to a pair of loci located in two neighbouring TADs. TADs are also functional blocks of chromosomes as enhancers and their cognate promoters are normally located in the same TAD, even if their genomic distance from each other can be as large as a megabase. The internal structure of TADs, causing their increased frequency of internal interactions, is not established yet. We survey here experimental studies investigating presence of supercoiling in interphase chromosomes. We also review numerical simulation studies testing whether transcription-induced supercoiling of chromatin fibres can explain how TADs are formed and how they can assure very efficient interactions between enhancers and their cognate promoters located in the same TAD.
Keywords
Chromatin, Chromosomes/chemistry, DNA, Superhelical, Enhancer Elements, Genetic, Models, Genetic, Promoter Regions, Genetic, Transcription, Genetic
Pubmed
Web of science
Open Access
Yes
Create date
13/11/2018 12:39
Last modification date
12/09/2019 5:10
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