Determination of 5,10,15,20-tetra-(m-hydroxyphenyl)chlorin in human plasma by high performance liquid chromatography

Details

Serval ID
serval:BIB_12B8AFD436B4
Type
Article: article from journal or magazin.
Collection
Publications
Title
Determination of 5,10,15,20-tetra-(m-hydroxyphenyl)chlorin in human plasma by high performance liquid chromatography
Journal
Biomedical Chromatography
Author(s)
Wang  Q., Ris  H. B., Altermatt  H. J., Reynolds  B., Stewart  J. C., Bonnett  R., Lim  C. K.
ISSN
0269-3879 (Print)
Publication state
Published
Issued date
02/1993
Volume
7
Number
1
Pages
45-7
Notes
Journal Article --- Old url value: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8431680 --- Old month value: Jan-Feb
Abstract
A high performance liquid chromatographic method for the determination of the photodynamic chemotherapeutic agent 5,10,15,20-tetra(m-hydroxyphenyl)chlorin (m-THPC) in human plasma following intravenous infusion is described. The procedure involves extraction of the drug in plasma with methanol/dimethyl sulphoxide (4:1 v/v) containing 5,10,15,20-tetra(p-hydroxyphenyl)chlorin as the internal standard and separation on a C18 reversed phase column with acetonitrile:0.1% trifluoroacetic acid (77:23 v/v) as the mobile phase. The drug was detected specifically and sensitively at its absorption maximum of 423 nm with a detection limit of 15 ng/mL (signal-to-noise ratio of 5). The intra- and inter-assay coefficients of variation (CV) on analysis of a plasma spiked with m-THPC (1 micrograms/mL) were 2.3 and 3.4% (n = 6), respectively.
Keywords
Antineoplastic Agents/administration & dosage/*blood Chromatography, High Pressure Liquid Humans Indicators and Reagents Infusions, Intravenous Mesoporphyrins/administration & dosage/*blood Mesothelioma/blood Reference Standards Solvents
Pubmed
Web of science
Create date
29/01/2008 13:00
Last modification date
20/08/2019 12:40
Usage data