Determination of 5,10,15,20-tetra-(m-hydroxyphenyl)chlorin in human plasma by high performance liquid chromatography
Détails
ID Serval
serval:BIB_12B8AFD436B4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Determination of 5,10,15,20-tetra-(m-hydroxyphenyl)chlorin in human plasma by high performance liquid chromatography
Périodique
Biomedical Chromatography
ISSN
0269-3879 (Print)
Statut éditorial
Publié
Date de publication
02/1993
Volume
7
Numéro
1
Pages
45-7
Notes
Journal Article --- Old url value: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8431680 --- Old month value: Jan-Feb
Résumé
A high performance liquid chromatographic method for the determination of the photodynamic chemotherapeutic agent 5,10,15,20-tetra(m-hydroxyphenyl)chlorin (m-THPC) in human plasma following intravenous infusion is described. The procedure involves extraction of the drug in plasma with methanol/dimethyl sulphoxide (4:1 v/v) containing 5,10,15,20-tetra(p-hydroxyphenyl)chlorin as the internal standard and separation on a C18 reversed phase column with acetonitrile:0.1% trifluoroacetic acid (77:23 v/v) as the mobile phase. The drug was detected specifically and sensitively at its absorption maximum of 423 nm with a detection limit of 15 ng/mL (signal-to-noise ratio of 5). The intra- and inter-assay coefficients of variation (CV) on analysis of a plasma spiked with m-THPC (1 micrograms/mL) were 2.3 and 3.4% (n = 6), respectively.
Mots-clé
Antineoplastic Agents/administration & dosage/*blood
Chromatography, High Pressure Liquid
Humans
Indicators and Reagents
Infusions, Intravenous
Mesoporphyrins/administration & dosage/*blood
Mesothelioma/blood
Reference Standards
Solvents
Pubmed
Web of science
Création de la notice
29/01/2008 13:00
Dernière modification de la notice
20/08/2019 12:40