A novel member of the interferon receptor family complements functionality of the murine interferon gamma receptor in human cells.

Details

Serval ID
serval:BIB_110080D02FA8
Type
Article: article from journal or magazin.
Collection
Publications
Title
A novel member of the interferon receptor family complements functionality of the murine interferon gamma receptor in human cells.
Journal
Cell
Author(s)
Hemmi S., Böhni R., Stark G., Di Marco F., Aguet M.
ISSN
0092-8674 (Print)
ISSN-L
0092-8674
Publication state
Published
Issued date
1994
Volume
76
Number
5
Pages
803-810
Language
english
Abstract
Expression of the human interferon gamma receptor (IFN-gamma R) in mouse cells is not sufficient to confer biological responsiveness to human IFN-gamma and vice versa. An additional species-specific component is required for signal transduction. We identified this cofactor by expression cloning in simian COS cells stably transfected with the nonfunctional murine IFN-gamma R and a IFN-gamma-inducible reporter construct encoding the human Tac antigen (interleukin-2 receptor alpha chain, CD25). A cDNA clone was obtained that, upon stable transfection, rendered human HEp-2 cells expressing the murine IFN-gamma R fully responsive to murine IFN-gamma. This cDNA encodes a novel 332 amino acid type I transmembrane protein that belongs to the IFN receptor family and that we designate IFN-gamma R beta chain.
Keywords
Amino Acid Sequence, Animals, Base Sequence, Chromosomes, Human, Pair 21, Cloning, Molecular, DNA, Complementary/genetics, Genes, Genetic Complementation Test, Humans, Mice, Molecular Sequence Data, Receptors, Interferon/chemistry, Receptors, Interferon/genetics, Sequence Alignment, Sequence Homology, Amino Acid
Pubmed
Web of science
Create date
28/01/2008 11:36
Last modification date
20/08/2019 12:38
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