A novel member of the interferon receptor family complements functionality of the murine interferon gamma receptor in human cells.
Details
Serval ID
serval:BIB_110080D02FA8
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A novel member of the interferon receptor family complements functionality of the murine interferon gamma receptor in human cells.
Journal
Cell
ISSN
0092-8674 (Print)
ISSN-L
0092-8674
Publication state
Published
Issued date
1994
Volume
76
Number
5
Pages
803-810
Language
english
Abstract
Expression of the human interferon gamma receptor (IFN-gamma R) in mouse cells is not sufficient to confer biological responsiveness to human IFN-gamma and vice versa. An additional species-specific component is required for signal transduction. We identified this cofactor by expression cloning in simian COS cells stably transfected with the nonfunctional murine IFN-gamma R and a IFN-gamma-inducible reporter construct encoding the human Tac antigen (interleukin-2 receptor alpha chain, CD25). A cDNA clone was obtained that, upon stable transfection, rendered human HEp-2 cells expressing the murine IFN-gamma R fully responsive to murine IFN-gamma. This cDNA encodes a novel 332 amino acid type I transmembrane protein that belongs to the IFN receptor family and that we designate IFN-gamma R beta chain.
Keywords
Amino Acid Sequence, Animals, Base Sequence, Chromosomes, Human, Pair 21, Cloning, Molecular, DNA, Complementary/genetics, Genes, Genetic Complementation Test, Humans, Mice, Molecular Sequence Data, Receptors, Interferon/chemistry, Receptors, Interferon/genetics, Sequence Alignment, Sequence Homology, Amino Acid
Pubmed
Web of science
Create date
28/01/2008 12:36
Last modification date
20/08/2019 13:38