Immunoglobulin classes in aquatic mammals. Characterization by serologic cross-reactivity, molecular size and binding of human free secretory component.

Details

Serval ID
serval:BIB_10CC83E29DC8
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Immunoglobulin classes in aquatic mammals. Characterization by serologic cross-reactivity, molecular size and binding of human free secretory component.
Journal
Journal of Immunology
Author(s)
Nash D.R., Mach J.P.
ISSN
0022-1767 (Print)
ISSN-L
0022-1767
Publication state
Published
Issued date
1971
Volume
107
Number
5
Pages
1424-1430
Language
english
Abstract
On the basis of serologic cross-reactivity, three immunoglobulin classes homologous to human IgG, IgM and IgA were identified in two species of acquatic mammal representing the orders Cetacea (dolphin) and Pinnipedea (sea lion). Molecular size was estimated by sucrose density gradient ultracentrifugation and Sephadex G-200 chromatography, indicating a 7S IgG, 19S IgM and heterogeneous serum IgA. Human secretory component was readily bound to the IgM of both species and to an apparently lesser extent to the larger molecular size populations of IgA. No binding was observed with IgG. Several antisera specific for human γ-chains gave a single precipitin line with the sea lion IgG but when made to react with dolphin serum produced two lines, suggesting the presence of two different subclasses of IgG in this species.
Keywords
Animals, Antigen-Antibody Reactions, Autoradiography, Centrifugation, Density Gradient, Chromatography, Chromatography, Gel, Cross Reactions, Dolphins/immunology, Humans, Immunodiffusion, Immunoelectrophoresis, Immunoglobulin A/analysis, Immunoglobulin G/analysis, Immunoglobulin M, Immunoglobulins/analysis, Iodine Isotopes, Pinnipedia/immunology, Species Specificity
Pubmed
Web of science
Create date
24/04/2013 9:55
Last modification date
20/08/2019 12:38
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