The collecting tubule of Amphiuma. I. Electrophysiological characterization

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Serval ID
serval:BIB_107D4433A4D4
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The collecting tubule of Amphiuma. I. Electrophysiological characterization
Journal
American Journal of Physiology
Author(s)
Hunter  M., Horisberger  J. D., Stanton  B., Giebisch  G.
ISSN
0002-9513 (Print)
Publication state
Published
Issued date
12/1987
Volume
253
Number
6 Pt 2
Pages
F1263-72
Notes
Journal Article
Research Support, U.S. Gov't, P.H.S. --- Old month value: Dec
Abstract
Single collecting tubules of Amphiuma kidneys were perfused in vitro to characterize their electrophysiological properties. The lumen-negative potential (-24 mV) was abolished by amiloride in the lumen and by ouabain in the bath. Ion substitution experiments in the lumen demonstrated the presence of a large sodium conductance in the apical cell membrane, but no evidence was obtained for a significant potassium or chloride conductance. Ion substitutions in the bath solution and the depolarizing effect of barium on the basolateral membrane potential demonstrated the presence of a large potassium conductance in the basolateral cell membrane. Measurements of dilution potentials in amiloride-treated tubules revealed a modest cation selectivity of the paracellular pathway. These results support a cell model in which sodium reabsorption occurs by electrodiffusion across the apical cell membrane and active transport across the basolateral cell membrane. The absence of a detectable potassium conductance in the apical cell membrane suggests that secretion of this ion cannot take place by diffusion from cell to lumen.
Keywords
Algorithms Amiloride/pharmacology Animals Barium/pharmacology Electric Conductivity Electrophysiology Female Kidney Tubules/*physiology Kidney Tubules, Collecting/*physiology Male Ouabain/pharmacology Reference Values Urodela/*physiology
Pubmed
Web of science
Create date
24/01/2008 12:38
Last modification date
20/08/2019 12:37
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