Activation of HTLV-I transcription in the presence of Tax is independent of the acetylation of CREB-2 (ATF-4).

Details

Serval ID
serval:BIB_100DCF882EC0
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Activation of HTLV-I transcription in the presence of Tax is independent of the acetylation of CREB-2 (ATF-4).
Journal
Virology
Author(s)
Gachon F., Devaux C., Mesnard J.M.
ISSN
0042-6822
Publication state
Published
Issued date
2002
Peer-reviewed
Oui
Volume
299
Number
2
Pages
271-278
Language
english
Abstract
We have previously demonstrated that the bZIP transcription factor CREB-2, also called ATF-4, trans-activates, in association with the viral protein Tax, the human T-cell leukemia virus type I (HTLV-I) promoter. In this study, we have examined whether CREB-2 acetylation affects transcriptional activation mediated by Tax. We present evidence that CREB-2 is acetylated in vitro and in vivo. CREB-2 is acetylated in two regions: the basic domain of the bZIP (from amino acid residue 270 to 300) and the short basic domain (from 342 to 351) located downstream from the bZIP. We also demonstrate that CREB-2 is acetylated by p300/CBP but not by p/CAF. Moreover, replacement of lysine by arginine in the basic domains decreases the trans-activating capacity of CREB-2. However, in the presence of Tax, the HTLV-I transcription remains fully activated by these CREB-2 mutants. Although we cannot totally exclude that the mutations could also affect CREB-2 structure and activity independent of acetylation, our results suggest that activation of the viral promoter in the presence of Tax is independent of the CREB-2 acetylation.
Keywords
Acetylation, Activating Transcription Factor 4, Amino Acid Sequence, Gene Products, tax/physiology, Human T-lymphotropic virus 1/genetics, Humans, Molecular Sequence Data, Nuclear Proteins/physiology, Trans-Activators/physiology, Transcription Factors/chemistry, Transcription Factors/metabolism, Transcriptional Activation
Pubmed
Web of science
Open Access
Yes
Create date
13/10/2009 9:07
Last modification date
20/08/2019 12:36
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