A large number of novel Ergtoxin-like genes and ERG K+-channels blocking peptides from scorpions of the genus Centruroides.

Details

Serval ID
serval:BIB_0EF608CDF7F3
Type
Article: article from journal or magazin.
Collection
Publications
Title
A large number of novel Ergtoxin-like genes and ERG K+-channels blocking peptides from scorpions of the genus Centruroides.
Journal
FEBS Letters
Author(s)
Corona M., Gurrola G.B., Merino E., Cassulini R.R., Valdez-Cruz N.A., García B., Ramírez-Domínguez M.E., Coronas F.I., Zamudio F.Z., Wanke E., Possani L.D.
ISSN
0014-5793
Publication state
Published
Issued date
2002
Peer-reviewed
Oui
Volume
532
Number
1-2
Pages
121-126
Language
english
Abstract
Twenty-three novel sequences similar to Ergtoxin (ErgTx) were obtained by direct sequencing of peptides or deduced from gene cloned using cDNAs of venomous glands of Centruroides (C.) elegans, C. exilicauda, C. gracilis, C. limpidus limpidus, C. noxius and C. sculpturatus. These peptides have from 42 to 47 amino acid residues cross-linked by four disulfide bridges. They share sequence similarities (60-98% compared with ErgTx1) and were shown to block ERG K(+)-channels of F-11 clone (N18TG-2xrat DRG) cultured cells. An unrooted phylogenetic tree analysis of these peptides showed that they conform at least five different subfamilies, of which three are novel subfamilies.
Keywords
Amino Acid Sequence, Animals, Cloning, Molecular, Electric Conductivity, Genes, Mice, Molecular Sequence Data, Peptides/genetics, Peptides/pharmacology, Phylogeny, Potassium Channel Blockers/classification, Potassium Channel Blockers/pharmacology, Potassium Channels/physiology, Scorpion Venoms/classification, Scorpion Venoms/genetics, Sequence Alignment, Tumor Cells, Cultured
Pubmed
Web of science
Open Access
Yes
Create date
10/03/2008 12:51
Last modification date
20/08/2019 13:35
Usage data