Thin chip microsprayer system coupled to quadrupole time-of-flight mass spectrometer for glycoconjugate analysis.

Details

Serval ID
serval:BIB_0EB3D7616A6A
Type
Article: article from journal or magazin.
Collection
Publications
Title
Thin chip microsprayer system coupled to quadrupole time-of-flight mass spectrometer for glycoconjugate analysis.
Journal
Lab on a Chip
Author(s)
Zamfir A.D., Lion N., Vukelic Z., Bindila L., Rossier J., Girault H.H., Peter-Katalinic J.
ISSN
1473-0197 (Print)
ISSN-L
1473-0189
Publication state
Published
Issued date
2005
Peer-reviewed
Oui
Volume
5
Number
3
Pages
298-307
Language
english
Notes
Publication types: Journal Article Publication Status: ppublish
Abstract
A thin chip polymer-based microsprayer has been coupled to a hybrid quadrupole time-of-flight mass spectrometer (QTOF MS) and introduced in carbohydrate research. The feasibility of the approach is demonstrated for mapping, sequencing and structural elucidation of glycoconjugates originating from human body fluids and tissues such as a glycopeptide mixture from normal human urine and an isolated and purified GT1 ganglioside fraction from normal adult human brain. The optimization procedure required by each glycoconjugate category is described and the advantages of the system in terms of flexibility and adaptability to QTOF MS, stability of the ESI MS signal, carbohydrate ionization and sequencing, sensitivity, speed of analysis and sample consumption are discussed.
Keywords
Amino Acids/analysis, Amino Acids/urine, Brain Chemistry, Cerebrospinal Fluid/chemistry, Gangliosides/analysis, Glycoconjugates/analysis, Glycoconjugates/urine, Glycopeptides/analysis, Glycopeptides/urine, Humans, Microchemistry/instrumentation, Microchemistry/methods, Microchip Analytical Procedures/instrumentation, Microchip Analytical Procedures/methods, Middle Aged, Nebulizers and Vaporizers, Reference Standards, Sensitivity and Specificity, Spectrometry, Mass, Electrospray Ionization/instrumentation
Pubmed
Web of science
Create date
09/11/2014 22:48
Last modification date
20/08/2019 12:35
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