Skeletal regeneration in the brittle star Amphiura filiformis.

Details

Serval ID
serval:BIB_0DF2817F232A
Type
Article: article from journal or magazin.
Collection
Publications
Title
Skeletal regeneration in the brittle star Amphiura filiformis.
Journal
Frontiers in Zoology
Author(s)
Czarkwiani A., Ferrario C., Dylus D.V., Sugni M., Oliveri P.
ISSN
1742-9994 (Electronic)
ISSN-L
1742-9994
Publication state
Published
Issued date
2016
Peer-reviewed
Oui
Volume
13
Pages
18
Language
english
Abstract
BACKGROUND: Brittle stars regenerate their whole arms post-amputation. Amphiura filiformis can now be used for molecular characterization of arm regeneration due to the availability of transcriptomic data. Previous work showed that specific developmental transcription factors known to take part in echinoderm skeletogenesis are expressed during adult arm regeneration in A. filiformis; however, the process of skeleton formation remained poorly understood. Here, we present the results of an in-depth microscopic analysis of skeletal morphogenesis during regeneration, using calcein staining, EdU labeling and in situ hybridization.
RESULTS: To better compare different samples, we propose a staging system for the early A. filiformis arm regeneration stages based on morphological landmarks identifiable in living animals and supported by histological analysis. We show that the calcified spicules forming the endoskeleton first appear very early during regeneration in the dermal layer of regenerates. These spicules then mature into complex skeletal elements of the differentiated arm during late regeneration. The mesenchymal cells in the dermal area express the skeletal marker genes Afi-c-lectin, Afi-p58b and Afi-p19; however, EdU labeling shows that these dermal cells do not proliferate.
CONCLUSIONS: A. filiformis arms regenerate through a consistent set of developmental stages using a distalization-intercalation mode, despite variability in regeneration rate. Skeletal elements form in a mesenchymal cell layer that does not proliferate and thus must be supplied from a different source. Our work provides the basis for future cellular and molecular studies of skeleton regeneration in brittle stars.
Pubmed
Web of science
Open Access
Yes
Create date
10/05/2016 17:40
Last modification date
20/08/2019 12:35
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