Interplay between the catabolite repression control protein Crc, Hfq and RNA in Hfq-dependent translational regulation in Pseudomonas aeruginosa.

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Version: Final published version
Serval ID
serval:BIB_0C70B756D940
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Interplay between the catabolite repression control protein Crc, Hfq and RNA in Hfq-dependent translational regulation in Pseudomonas aeruginosa.
Journal
Nucleic acids research
Author(s)
Sonnleitner E., Wulf A., Campagne S., Pei X.Y., Wolfinger M.T., Forlani G., Prindl K., Abdou L., Resch A., Allain F.H., Luisi B.F., Urlaub H., Bläsi U.
ISSN
1362-4962 (Electronic)
ISSN-L
0305-1048
Publication state
Published
Issued date
16/02/2018
Peer-reviewed
Oui
Volume
46
Number
3
Pages
1470-1485
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
In Pseudomonas aeruginosa the RNA chaperone Hfq and the catabolite repression control protein (Crc) act as post-transcriptional regulators during carbon catabolite repression (CCR). In this regard Crc is required for full-fledged Hfq-mediated translational repression of catabolic genes. RNAseq based transcriptome analyses revealed a significant overlap between the Crc and Hfq regulons, which in conjunction with genetic data supported a concerted action of both proteins. Biochemical and biophysical approaches further suggest that Crc and Hfq form an assembly in the presence of RNAs containing A-rich motifs, and that Crc interacts with both, Hfq and RNA. Through these interactions, Crc enhances the stability of Hfq/Crc/RNA complexes, which can explain its facilitating role in Hfq-mediated translational repression. Hence, these studies revealed for the first time insights into how an interacting protein can modulate Hfq function. Moreover, Crc is shown to interfere with binding of a regulatory RNA to Hfq, which bears implications for riboregulation. These results are discussed in terms of a working model, wherein Crc prioritizes the function of Hfq toward utilization of favored carbon sources.
Keywords
Bacterial Proteins/chemistry, Bacterial Proteins/genetics, Bacterial Proteins/metabolism, Binding Sites, Bordetella pertussis/genetics, Bordetella pertussis/metabolism, Carbohydrate Metabolism/genetics, Catabolite Repression, Escherichia coli/genetics, Escherichia coli/metabolism, Gene Expression Regulation, Bacterial, Host Factor 1 Protein/chemistry, Host Factor 1 Protein/genetics, Host Factor 1 Protein/metabolism, Kinetics, Models, Molecular, Nucleotide Motifs, Protein Binding, Protein Biosynthesis, Protein Interaction Domains and Motifs, Protein Structure, Secondary, Pseudomonas aeruginosa/genetics, Pseudomonas aeruginosa/metabolism, RNA, Bacterial/chemistry, RNA, Bacterial/genetics, RNA, Bacterial/metabolism, Regulon, Repressor Proteins/chemistry, Repressor Proteins/genetics, Repressor Proteins/metabolism, Transcriptome
Pubmed
Web of science
Open Access
Yes
Create date
11/01/2018 18:28
Last modification date
20/08/2019 13:33
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