Gene duplication and neofunctionalization: POLR3G and POLR3GL.

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State: Public
Version: Final published version
Serval ID
serval:BIB_0AEC073C49B3
Type
Article: article from journal or magazin.
Collection
Publications
Title
Gene duplication and neofunctionalization: POLR3G and POLR3GL.
Journal
Genome Research
Author(s)
Renaud M., Praz V., Vieu E., Florens L., Washburn M.P., L'hôte P., Hernandez N.
ISSN
1549-5469 (Electronic)
ISSN-L
1088-9051
Publication state
Published
Issued date
2014
Volume
24
Number
1
Pages
37-51
Language
english
Abstract
RNA polymerase III (Pol III) occurs in two versions, one containing the POLR3G subunit and the other the closely related POLR3GL subunit. It is not clear whether these two Pol III forms have the same function, in particular whether they recognize the same target genes. We show that the POLR3G and POLR3GL genes arose from a DNA-based gene duplication, probably in a common ancestor of vertebrates. POLR3G- as well as POLR3GL-containing Pol III are present in cultured cell lines and in normal mouse liver, although the relative amounts of the two forms vary, with the POLR3G-containing Pol III relatively more abundant in dividing cells. Genome-wide chromatin immunoprecipitations followed by high-throughput sequencing (ChIP-seq) reveal that both forms of Pol III occupy the same target genes, in very constant proportions within one cell line, suggesting that the two forms of Pol III have a similar function with regard to specificity for target genes. In contrast, the POLR3G promoter-not the POLR3GL promoter-binds the transcription factor MYC, as do all other promoters of genes encoding Pol III subunits. Thus, the POLR3G/POLR3GL duplication did not lead to neo-functionalization of the gene product (at least with regard to target gene specificity) but rather to neo-functionalization of the transcription units, which acquired different mechanisms of regulation, thus likely affording greater regulation potential to the cell.
Pubmed
Web of science
Open Access
Yes
Create date
23/01/2014 9:43
Last modification date
20/08/2019 13:32
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