Matrix-assisted laser desorption ionization-time of flight mass spectrometry for direct bacterial identification from positive blood culture pellets.

Details

Serval ID
serval:BIB_0A252319C70B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Matrix-assisted laser desorption ionization-time of flight mass spectrometry for direct bacterial identification from positive blood culture pellets.
Journal
Journal of Clinical Microbiology
Author(s)
Prod'hom Guy, Bizzini Alain, Durussel Christian, Bille Jacques, Greub Gilbert
ISSN
1098-660X[electronic], 0095-1137[linking]
Publication state
Published
Issued date
2010
Volume
48
Number
4
Pages
1481-1483
Language
english
Abstract
An ammonium chloride erythrocyte-lysing procedure was used to prepare a bacterial pellet from positive blood cultures for direct matrix-assisted laser desorption-ionization time of flight (MALDI-TOF) mass spectrometry analysis. Identification was obtained for 78.7% of the pellets tested. Moreover, 99% of the MALDI-TOF identifications were congruent at the species level when considering valid scores. This fast and accurate method is promising.
Pubmed
Web of science
Open Access
Yes
Create date
21/04/2010 9:48
Last modification date
20/08/2019 12:32
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