Matrix-assisted laser desorption ionization-time of flight mass spectrometry for direct bacterial identification from positive blood culture pellets.
Details
Serval ID
serval:BIB_0A252319C70B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Matrix-assisted laser desorption ionization-time of flight mass spectrometry for direct bacterial identification from positive blood culture pellets.
Journal
Journal of Clinical Microbiology
ISSN
1098-660X[electronic], 0095-1137[linking]
Publication state
Published
Issued date
2010
Volume
48
Number
4
Pages
1481-1483
Language
english
Abstract
An ammonium chloride erythrocyte-lysing procedure was used to prepare a bacterial pellet from positive blood cultures for direct matrix-assisted laser desorption-ionization time of flight (MALDI-TOF) mass spectrometry analysis. Identification was obtained for 78.7% of the pellets tested. Moreover, 99% of the MALDI-TOF identifications were congruent at the species level when considering valid scores. This fast and accurate method is promising.
Pubmed
Web of science
Open Access
Yes
Create date
21/04/2010 10:48
Last modification date
20/08/2019 13:32