Ly 2 positive cytotoxic T lymphocytes, whether specific or non-specific in lytic activity, may express a large, granular, vacuolated lymphocyte morphology.

Details

Serval ID
serval:BIB_084687BA5C8D
Type
Article: article from journal or magazin.
Collection
Publications
Title
Ly 2 positive cytotoxic T lymphocytes, whether specific or non-specific in lytic activity, may express a large, granular, vacuolated lymphocyte morphology.
Journal
Australian Journal of Experimental Biology and Medical Science
Author(s)
Wilson A., Scollay R., Abbot A.P., Shortman K.
ISSN
0004-945X (Print)
ISSN-L
0004-945X
Publication state
Published
Issued date
1984
Volume
62 ( Pt 4)
Pages
381-401
Language
english
Abstract
Cytotoxic clones consisting entirely of large, vacuolated granular lymphocytes (LGL) are produced with high frequency when individual purified mature Ly 2+ T cells are cultured at limit dilution in the presence of concanavalin A and irradiated spleen filler cells. Similar cells are produced in cultures of Ly 2- T cells but in lower proportion, the level of granular lymphocytes amongst the largely non-cytotoxic, Ly 2- L3T4+ product cells being only 20%. A proportion (15%) of LGL are also found in conventional mixed lymphocyte cultures. In the limit-dilution cultures of Ly 2+ T cells the LGL originate from the single precursor cell and not from the irradiated filler cells. They bear the markers expected of active cytotoxic T lymphocytes (Thy 1++; PNA++; Ly 1+; Ly 2++; L3T4-). Their DNA and chromosome complement appear normal. The granules within the cells are heterogeneous in form, and some resemble in ultrastructure those described in certain cytotoxic, suppressor and NK-cell lines. The granules do not show mast cell staining characteristics. The LGL are not phagocytic. Although LGL are associated with nonspecific cytotoxicity late in culture, the first granular cells appear early (day 3) and dominate in the clones at times when cytolysis is specific. LGL appear to be the normal morphological form of cytotoxic T lymphocytes when grown under these conditions.
Keywords
Animals, Antigens, Ly, Clone Cells/immunology, Clone Cells/ultrastructure, Cytoplasmic Granules/ultrastructure, DNA/analysis, Lymphocyte Culture Test, Mixed, Male, Mice, Mice, Inbred C57BL, Mice, Inbred CBA, T-Lymphocytes, Cytotoxic/analysis, T-Lymphocytes, Cytotoxic/immunology, Time Factors, Vacuoles/ultrastructure
Pubmed
Web of science
Create date
10/04/2013 10:29
Last modification date
20/08/2019 12:30
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