Transcription-induced supercoiling explains formation of self-interacting chromatin domains in S. pombe.

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Version: Final published version
Serval ID
serval:BIB_0678888C73AE
Type
Article: article from journal or magazin.
Collection
Publications
Title
Transcription-induced supercoiling explains formation of self-interacting chromatin domains in S. pombe.
Journal
Nucleic Acids Research
Author(s)
Benedetti F., Racko D., Dorier J., Burnier Y., Stasiak A.
ISSN
1362-4962 (Electronic)
ISSN-L
0305-1048
Publication state
Published
Issued date
2017
Peer-reviewed
Oui
Volume
45
Number
17
Pages
9850-9859
Language
english
Abstract
The question of how self-interacting chromatin domains in interphase chromosomes are structured and generated dominates current discussions on eukaryotic chromosomes. Numerical simulations using standard polymer models have been helpful in testing the validity of various models of chromosome organization. Experimental contact maps can be compared with simulated contact maps and thus verify how good is the model. With increasing resolution of experimental contact maps, it became apparent though that active processes need to be introduced into models to recapitulate the experimental data. Since transcribing RNA polymerases are very strong molecular motors that induce axial rotation of transcribed DNA, we present here models that include such rotational motors. We also include into our models swivels and sites for intersegmental passages that account for action of DNA topoisomerases releasing torsional stress. Using these elements in our models, we show that transcription-induced supercoiling generated in the regions with divergent-transcription and supercoiling relaxation occurring between these regions are sufficient to explain formation of self-interacting chromatin domains in chromosomes of fission yeast (S. pombe).

Keywords
Biomechanical Phenomena, Chromatin/chemistry, Chromatin/metabolism, Chromosomes, Fungal/chemistry, Chromosomes, Fungal/metabolism, DNA Topoisomerases/chemistry, DNA Topoisomerases/genetics, DNA Topoisomerases/metabolism, DNA, Fungal/chemistry, DNA, Fungal/genetics, DNA, Fungal/metabolism, DNA, Superhelical/chemistry, DNA, Superhelical/genetics, DNA, Superhelical/metabolism, DNA-Directed RNA Polymerases/chemistry, DNA-Directed RNA Polymerases/genetics, DNA-Directed RNA Polymerases/metabolism, Gene Expression Regulation, Fungal, Molecular Dynamics Simulation, Rotation, Schizosaccharomyces/genetics, Schizosaccharomyces/metabolism, Transcription, Genetic
Pubmed
Web of science
Open Access
Yes
Create date
09/10/2017 13:45
Last modification date
20/08/2019 13:28
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