2C-Cas9: a versatile tool for clonal analysis of gene function.

Details

Serval ID
serval:BIB_066EF01D78EF
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
2C-Cas9: a versatile tool for clonal analysis of gene function.
Journal
Genome Research
Author(s)
Di Donato V., De Santis F., Auer T.O., Testa N., Sánchez-Iranzo H., Mercader N., Concordet J.P., Del Bene F.
ISSN
1549-5469 (Electronic)
ISSN-L
1088-9051
Publication state
Published
Issued date
2016
Volume
26
Number
5
Pages
681-692
Language
english
Abstract
CRISPR/Cas9-mediated targeted mutagenesis allows efficient generation of loss-of-function alleles in zebrafish. To date, this technology has been primarily used to generate genetic knockout animals. Nevertheless, the study of the function of certain loci might require tight spatiotemporal control of gene inactivation. Here, we show that tissue-specific gene disruption can be achieved by driving Cas9 expression with the Gal4/UAS system. Furthermore, by combining the Gal4/UAS and Cre/loxP systems, we establish a versatile tool to genetically label mutant cell clones, enabling their phenotypic analysis. Our technique has the potential to be applied to diverse model organisms, enabling tissue-specific loss-of-function and phenotypic characterization of live and fixed tissues.
Pubmed
Web of science
Open Access
Yes
Create date
27/05/2016 8:58
Last modification date
20/08/2019 12:28
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