Detection of p53 gene mutation in cancer tissues by nonradioactive direct sequencing

Details

Serval ID
serval:BIB_061057DE35B9
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Detection of p53 gene mutation in cancer tissues by nonradioactive direct sequencing
Journal
PCR Methods and Applications
Author(s)
Bautista  D., Chaubert  P., Bertoncini  J., Benhattar  J.
ISSN
1054-9803 (Print)
Publication state
Published
Issued date
1994
Volume
4
Number
2
Pages
76-79
Notes
PT - Journal Article PT - Research Support, Non-U.S. Gov't
Abstract
A simple procedure for direct sequencing of double-stranded PCR products by the dideoxy-termination method has been developed using biotinylated sequencing primers. Sequences of the p53 gene have been obtained from DNA extracted from frozen and formalin-fixed paraffin-embedded cancer tissues. Detection of sequencing ladders was done with chemiluminescent or colorimetric techniques. Both are highly sensitive, but colorimetric detection is less prone to diffusion artifacts, which are common in G-rich regions. Use of this nonradioactive PCR-sequencing protocol allowed rapid and simple determination of p53 gene alterations in human tumors
Keywords
Base Sequence/Chemiluminescent Measurements/Colorimetry/DNA Primers/Electrophoresis,Agar Gel/Genes,p53/Humans/Molecular Sequence Data/Mutation/Neoplasms/genetics/Polymerase Chain Reaction/Sequence Analysis,DNA/methods/Research
Pubmed
Create date
29/01/2008 18:34
Last modification date
20/08/2019 12:28
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