Scaf1 promotes respiratory supercomplexes and metabolic efficiency in zebrafish

Details

Ressource 1Download: Garcia_Poyatos_EMBOreports_2020.pdf (2766.03 [Ko])
State: Public
Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_05E1F4028C2C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Scaf1 promotes respiratory supercomplexes and metabolic efficiency in zebrafish
Journal
EMBO reports
Author(s)
García-Poyatos Carolina, Cogliati Sara, Calvo Enrique, Hernansanz-Agustín Pablo, Lagarrigue Sylviane, Magni Ricardo, Botos Marius, Langa Xavier, Amati Francesca, Vázquez Jesús, Mercader Nadia, Enríquez José Antonio
ISSN
1469-3178 (Electronic)
ISSN-L
1469-221X
Publication state
Published
Issued date
03/07/2020
Peer-reviewed
Oui
Volume
21
Number
7
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
The oxidative phosphorylation (OXPHOS) system is a dynamic system in which the respiratory complexes coexist with super-assembled quaternary structures called supercomplexes (SCs). The physiological role of SCs is still disputed. Here, we used zebrafish to study the relevance of respiratory SCs. We combined immunodetection analysis and deep data-independent proteomics to characterize these structures and found similar SCs to those described in mice, as well as novel SCs including III <sub>2</sub> + IV <sub>2</sub> , I + IV, and I + III <sub>2 </sub> + IV <sub>2</sub> . To study the physiological role of SCs, we generated two null allele zebrafish lines for supercomplex assembly factor 1 (scaf1). scaf1 <sup>-/-</sup> fish displayed altered OXPHOS activity due to the disrupted interaction of complexes III and IV. scaf1 <sup>-/-</sup> fish were smaller in size and showed abnormal fat deposition and decreased female fertility. These physiological phenotypes were rescued by doubling the food supply, which correlated with improved bioenergetics and alterations in the metabolic gene expression program. These results reveal that SC assembly by Scaf1 modulates OXPHOS efficiency and allows the optimization of metabolic resources.
Keywords
Genetics, Biochemistry, Molecular Biology
Pubmed
Open Access
Yes
Funding(s)
Swiss National Science Foundation / 31003A159721
Swiss National Science Foundation / 320030_170062
Create date
04/06/2020 21:37
Last modification date
23/10/2020 5:23
Usage data