Targeting of secretory IgA to Peyer's patch dendritic and T cells after transport by intestinal M cells.

Details

Serval ID
serval:BIB_059C9FA09817
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Targeting of secretory IgA to Peyer's patch dendritic and T cells after transport by intestinal M cells.
Journal
Journal of Immunology
Author(s)
Rey J., Garin N., Spertini F., Corthésy B.
ISSN
0022-1767[print], 0022-1767[linking]
Publication state
Published
Issued date
2004
Volume
172
Number
5
Pages
3026-3033
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Abstract
In addition to being instrumental to the protection of mucosal epithelia, secretory IgA (SIgA) adheres to and is transported by intestinal Peyer's patch (PP) M cells. The possible functional reason for this transport is unknown. We have thus examined in mice the outcome of SIgA delivered from the intestinal lumen to the cells present in the underlying organized mucosa-associated lymphoreticular tissue. We show selective association of SIgA with dendritic cells and CD4(+) T and B lymphocytes recovered from PP in vitro. In vivo, exogenously delivered SIgA is able to enter into multiple PP lining the intestine. In PP, SIgA associates with and is internalized by dendritic cells in the subepithelial dome region, whereas the interaction with CD4(+) T cells is limited to surface binding. Interaction between cells and SIgA is mediated by the IgA moiety and occurs for polymeric and monomeric molecular forms. Thus, although immune exclusion represents the main function of SIgA, transport of the Ab by M cells might promote Ag sampling under neutralizing conditions essential to the homeostasis of mucosal surfaces.
Keywords
Animals, Binding Sites, Antibody, Cell Separation, Dendritic Cells/immunology, Dendritic Cells/metabolism, Endocytosis/immunology, Flow Cytometry, Immunoglobulin A, Secretory/administration &amp, dosage, Immunoglobulin A, Secretory/metabolism, Injections, Intraperitoneal, Intestinal Mucosa/cytology, Intestinal Mucosa/immunology, Ligation, Mice, Peyer's Patches/cytology, Peyer's Patches/immunology, Protein Binding/immunology, Protein Transport/immunology, Receptors, Antigen, B-Cell/metabolism, Secretory Component/metabolism, T-Lymphocyte Subsets/immunology, T-Lymphocyte Subsets/metabolism
Pubmed
Web of science
Create date
25/01/2008 14:53
Last modification date
20/08/2019 12:27
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