Competition between SsrA tagging and translational termination at weak stop codons in Escherichia coli.

Details

Serval ID
serval:BIB_04ADCB482A32
Type
Article: article from journal or magazin.
Collection
Publications
Title
Competition between SsrA tagging and translational termination at weak stop codons in Escherichia coli.
Journal
Molecular Microbiology
Author(s)
Collier J., Binet E., Bouloc P.
ISSN
0950-382X
Publication state
Published
Issued date
2002
Peer-reviewed
Oui
Volume
45
Number
3
Pages
745-754
Language
english
Abstract
SsrA is a tmRNA involved in tagging polypeptides on stalled ribosomes. The resulting fusion proteins are then degraded. We purified endogenous SsrA-tagged proteins by means of a genetically engineered SsrA and identified some of them. Analysis of the proteins suggested that they are tagged at their C-terminal extremities. One of them, ribokinase, is expressed from a messenger with a poorly efficient stop codon, leading to translational recoding events. A change in the ribokinase coding sequence from a weak to a strong translational stop sequence (UGAc to UAAu) annihilated SsrA tagging. Translational termination by UGA recruits the translational release factor (RF) 2. We observed that SsrA tagging of ribokinase was inversely correlated with RF2 activity, revealing a dynamic competition between translational termination and SsrA tagging.
Keywords
Codon, Terminator, Escherichia coli, Escherichia coli Proteins, Gene Expression Regulation, Bacterial, Phosphotransferases (Alcohol Group Acceptor), Plasmids, Protein Biosynthesis, RNA Processing, Post-Transcriptional, RNA, Bacterial, RNA, Messenger, Regulatory Sequences, Nucleic Acid, Ribosomes
Pubmed
Web of science
Open Access
Yes
Create date
27/02/2009 16:47
Last modification date
08/05/2019 13:52
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