Validation of an analytical method for nitrous oxide (N2O) laughing gas by headspace gas chromatography coupled to mass spectrometry (HS-GC-MS): Forensic application to a lethal intoxication.

Details

Serval ID
serval:BIB_03F71641295D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Validation of an analytical method for nitrous oxide (N2O) laughing gas by headspace gas chromatography coupled to mass spectrometry (HS-GC-MS): Forensic application to a lethal intoxication.
Journal
Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Author(s)
Giuliani N., Beyer J., Augsburger M., Varlet V.
ISSN
1873-376X (Electronic)
ISSN-L
1570-0232
Publication state
Published
Issued date
2015
Peer-reviewed
Oui
Volume
983-984
Pages
90-93
Language
english
Notes
Publication types: Journal Article Publication Status: ppublish
Abstract
Drug abuse is a widespread problem affecting both teenagers and adults. Nitrous oxide is becoming increasingly popular as an inhalation drug, causing harmful neurological and hematological effects. Some gas chromatography-mass spectrometry (GC-MS) methods for nitrous oxide measurement have been previously described. The main drawbacks of these methods include a lack of sensitivity for forensic applications; including an inability to quantitatively determine the concentration of gas present. The following study provides a validated method using HS-GC-MS which incorporates hydrogen sulfide as a suitable internal standard allowing the quantification of nitrous oxide. Upon analysis, sample and internal standard have similar retention times and are eluted quickly from the molecular sieve 5Å PLOT capillary column and the Porabond Q column therefore providing rapid data collection whilst preserving well defined peaks. After validation, the method has been applied to a real case of N2O intoxication indicating concentrations in a mono-intoxication.
Pubmed
Web of science
Create date
27/01/2015 15:53
Last modification date
20/08/2019 13:25
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