Developmental expression of glial fibrillary acidic protein and glutamine synthetase in serum-free aggregating cell cultures of fetal rat telencephalon.

Details

Serval ID
serval:BIB_039FBE62E723
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Developmental expression of glial fibrillary acidic protein and glutamine synthetase in serum-free aggregating cell cultures of fetal rat telencephalon.
Journal
Developmental Neuroscience
Author(s)
Monnet-Tschudi F., Eng L.F., Matthieu J.M., Honegger P.
ISSN
0378-5866 (Print)
ISSN-L
0378-5866
Publication state
Published
Issued date
1988
Volume
10
Number
3
Pages
165-172
Language
english
Abstract
Serum-free aggregating cell cultures of fetal rat telencephalon were examined by a combined biochemical and double-labeling immunocytochemical study for the developmental expression of glial fibrillary acidic protein (GFAP) and glutamine synthetase (GS). It was found that these two astroglial markers are co-expressed at different developmental stages in vitro. During the phase of cellular maturation (i.e. between days 14 and 34), GFAP levels and GS activity increase rapidly and in parallel. At the same time, the number of immunoreactive cells increase while the long and thick processes staining in early cultures gradually disappear. The present results demonstrate that in this particular cell culture system only one type of astrocytes develops which expresses both GFAP and GS and which attains a relatively high degree of maturation.
Keywords
Animals, Astrocytes/cytology, Astrocytes/metabolism, Cell Aggregation, Cells, Cultured, Glial Fibrillary Acidic Protein/metabolism, Glial Fibrillary Acidic Protein/physiology, Glutamate-Ammonia Ligase/metabolism, Glutamate-Ammonia Ligase/physiology, Immunohistochemistry, Rats, Telencephalon/cytology, Telencephalon/embryology, Time Factors
Pubmed
Web of science
Create date
24/01/2008 13:11
Last modification date
20/08/2019 12:25
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