Differences between calcium-stimulated and storage-induced erythrocyte-derived microvesicles.

Details

Serval ID
serval:BIB_01FFD69B83F7
Type
Article: article from journal or magazin.
Collection
Publications
Title
Differences between calcium-stimulated and storage-induced erythrocyte-derived microvesicles.
Journal
Transfusion and Apheresis Science
Author(s)
Prudent M., Crettaz D., Delobel J., Seghatchian J., Tissot J.D., Lion N.
ISSN
1473-0502 (Print)
ISSN-L
1473-0502
Publication state
Published
Issued date
2015
Peer-reviewed
Oui
Volume
53
Number
2
Pages
153-158
Language
english
Abstract
Microvesicles (MVs), or microparticles, are a complex, dynamic and functional part of cells. Red blood cell (RBC)-derived MVs are naturally produced in vivo (during normal aging processes or in several diseases) as well as ex vivo during cold storage of RBCs, or in vitro by ATP depletion or treatment with Ca(2+) and calcium ionophore. All these MVs are equivalently classified according to their size and/or surface markers. Nevertheless, their content in proteins can differ and a few differences in terms of lipid raft proteins, notably stomatin and flotillin-2, have been reported. Based on two-dimensional gel electrophoreses, the present study highlights the differences between MVs induced during storage of RBCs (storage-MVs) and MVs stimulated by Ca(2+) entry (Ca-MVs). Upon treatment, Ca-MVs are formed following a clear recruitment of Ca(2+)-binding proteins (sorcin, grancalcin, PDCD6) and particularly annexins (4 and 5). Therefore, it emerges that different molecular pathways are available to produce similar MVs by disturbing the membrane/cytoskeleton interactions. Interestingly, these differences provide non-negligible pieces of information on the parent cells, and the mechanisms and modes of actions involved in the formation of MVs. In addition to biophysical characterization, protein analysis is important to classify these cellular corpuscles and evaluate their potential impacts in diseases or transfusion medicine.
Pubmed
Create date
10/03/2016 9:34
Last modification date
20/08/2019 12:24
Usage data