In vivo observation of lactate methyl proton magnetization transfer in rat C6 glioma.

Details

Serval ID
serval:BIB_012BCA411E0F
Type
Article: article from journal or magazin.
Collection
Publications
Title
In vivo observation of lactate methyl proton magnetization transfer in rat C6 glioma.
Journal
Magnetic Resonance in Medicine
Author(s)
Luo Y., Rydzewski J., de Graaf R.A., Gruetter R., Garwood M., Schleich T.
ISSN
0740-3194 (Print)
ISSN-L
0740-3194
Publication state
Published
Issued date
1999
Volume
41
Number
4
Pages
676-685
Language
english
Notes
Publication types: Journal Article ; Research Support, U.S. Gov't, P.H.S.Publication Status: ppublish
Abstract
Magnetic resonance spectroscopy (MRS) measurements of the lactate methyl proton in rat brain C6 glioma tissue acquired in the presence of an off-resonance irradiation field, analyzed using coupled Bloch equation formalism assuming two spin pools, demonstrated the occurrence of magnetization transfer. Quantitative analysis revealed that a very small fraction of lactate (f = 0.0012) is rotationally immobilized despite a large magnetization transfer effect. Off-resonance rotating frame spin-lattice relaxation studies demonstrated that deuterated lactate binds to bovine serum albumin and the proteins present in human plasma, thereby providing a possible physical basis for the observed magnetization transfer effect. These results demonstrate that partial or complete saturation of the motionally restricted lactate pool (as well as other metabolites) by the application of an off-resonance irradiation field, such as that used for water presaturation, can lead to a substantial decrease in resonance intensity by way of magnetization transfer effects, resulting in quantitation errors.
Keywords
Animals, Glioma/metabolism, Lactates/analysis, Magnetic Resonance Spectroscopy/methods, Male, Methylation, Models, Theoretical, Rats, Rats, Inbred F344
Pubmed
Web of science
Create date
04/08/2010 15:28
Last modification date
20/08/2019 12:23
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