In vivo observation of lactate methyl proton magnetization transfer in rat C6 glioma.

Détails

ID Serval
serval:BIB_012BCA411E0F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
In vivo observation of lactate methyl proton magnetization transfer in rat C6 glioma.
Périodique
Magnetic Resonance in Medicine
Auteur⸱e⸱s
Luo Y., Rydzewski J., de Graaf R.A., Gruetter R., Garwood M., Schleich T.
ISSN
0740-3194 (Print)
ISSN-L
0740-3194
Statut éditorial
Publié
Date de publication
1999
Volume
41
Numéro
4
Pages
676-685
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, U.S. Gov't, P.H.S.Publication Status: ppublish
Résumé
Magnetic resonance spectroscopy (MRS) measurements of the lactate methyl proton in rat brain C6 glioma tissue acquired in the presence of an off-resonance irradiation field, analyzed using coupled Bloch equation formalism assuming two spin pools, demonstrated the occurrence of magnetization transfer. Quantitative analysis revealed that a very small fraction of lactate (f = 0.0012) is rotationally immobilized despite a large magnetization transfer effect. Off-resonance rotating frame spin-lattice relaxation studies demonstrated that deuterated lactate binds to bovine serum albumin and the proteins present in human plasma, thereby providing a possible physical basis for the observed magnetization transfer effect. These results demonstrate that partial or complete saturation of the motionally restricted lactate pool (as well as other metabolites) by the application of an off-resonance irradiation field, such as that used for water presaturation, can lead to a substantial decrease in resonance intensity by way of magnetization transfer effects, resulting in quantitation errors.
Mots-clé
Animals, Glioma/metabolism, Lactates/analysis, Magnetic Resonance Spectroscopy/methods, Male, Methylation, Models, Theoretical, Rats, Rats, Inbred F344
Pubmed
Web of science
Création de la notice
04/08/2010 15:28
Dernière modification de la notice
20/08/2019 12:23
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