Kinetics of cytokine expression during primary human immunodeficiency virus type 1 infection

Details

Serval ID
serval:BIB_0101B41AE49B
Type
Article: article from journal or magazin.
Collection
Publications
Title
Kinetics of cytokine expression during primary human immunodeficiency virus type 1 infection
Journal
Proceedings of the National Academy of Sciences of the United States of America
Author(s)
Graziosi  C., Gantt  K. R., Vaccarezza  M., Demarest  J. F., Daucher  M., Saag  M. S., Shaw  G. M., Quinn  T. C., Cohen  O. J., Welbon  C. C., Pantaleo  G., Fauci  A. S.
ISSN
0027-8424 (Print)
Publication state
Published
Issued date
04/1996
Volume
93
Number
9
Pages
4386-91
Notes
Journal Article --- Old month value: Apr 30
Abstract
In the present study, we have determined the kinetics of constitutive expression of a panel of cytokines [interleukin (IL) 2, IL-4, IL-6, IL-10, interferon gamma (IFN-gamma), and tumor necrosis factor alpha (TNF-alpha)] in sequential peripheral blood mononuclear cell samples from nine individuals with primary human immunodeficiency virus infection. Expression of IL-2 and IL-4 was barely detected in peripheral blood mononuclear cells. However, substantial levels of IL-2 expression were found in mononuclear cells isolated from lymph node. Expression of IL-6 was detected in only three of nine patients, and IL-6 expression was observed when transition from the acute to the chronic phase had already occurred. Expression of IL-10 and TNF-alpha was consistently observed in all patients tested, and levels of both cytokines were either stable or progressively increased over time. Similar to IL-10 and TNF-alpha, IFN-gamma expression was detected in all patients; however, in five of nine patients, IFN-gamma expression peaked very early during primary infection. The early peak in IFN-gamma expression coincided with oligoclonal expansions of CD8+ T cells in five of six patients, and CD8+ T cells mostly accounted for the expression of this cytokine. These results indicate that high levels of expression of proinflammatory cytokines are associated with primary infection and that the cytokine response during this phase of infection is strongly influenced by oligoclonal expansions of CD8+ T cells.
Keywords
Cells, Cultured Cytokines/*biosynthesis Gene Expression Regulation, Viral HIV Infections/*immunology *Hiv-1 Humans Interferon Type II/biosynthesis Interleukin-10/biosynthesis Interleukin-2/biosynthesis Interleukin-4/biosynthesis Interleukin-6/biosynthesis Kinetics Lymph Nodes/immunology Lymphocytes/*immunology RNA, Messenger/analysis/biosynthesis Receptors, Antigen, T-Cell, alpha-beta T-Lymphocyte Subsets/immunology Time Factors Tumor Necrosis Factor-alpha/biosynthesis
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 15:58
Last modification date
08/05/2019 13:40
Usage data