serval:BIB_F914645B5C7B
High-density single cell mRNA sequencing to characterize circulating tumor cells in hepatocellular carcinoma.
10.1038/s41598-018-30047-y
000440411900019
30068984
D'Avola
D.
author
Villacorta-Martin
C.
author
Martins-Filho
S.N.
author
Craig
A.
author
Labgaa
I.
author
von Felden
J.
author
Kimaada
A.
author
Bonaccorso
A.
author
Tabrizian
P.
author
Hartmann
B.M.
author
Sebra
R.
author
Schwartz
M.
author
Villanueva
A.
author
article
2018-08-01
Scientific reports
2045-2322
2045-2322
journal
8
1
11570
Patients with hepatocellular carcinoma (HCC) release tumor cells to the bloodstream, which can be detected using cell surface markers. Despite numerous reports suggest a direct correlation between the number of circulating tumor cells (CTCs) and poor clinical outcomes, few studies have provided a thorough molecular characterization of CTCs. Due to the limited access to tissue samples in patients at advanced stages of HCC, it is crucial to develop new technologies to identify HCC cancer drivers in routine clinical conditions. Here, we describe a method that sequentially combines image flow cytometry and high density single-cell mRNA sequencing to identify CTCs in HCC patients. Genome wide expression profiling of CTCs using this approach demonstrates CTC heterogeneity and helps detect known oncogenic drivers in HCC such as IGF2. This integrated approach provides a novel tool for biomarker development in HCC using liquid biopsy.
Adult
Aged
Carcinoma, Hepatocellular/pathology
Female
Flow Cytometry
Gene Expression Profiling
Humans
Male
Middle Aged
Neoplastic Cells, Circulating/chemistry
RNA, Messenger/analysis
RNA, Messenger/genetics
Sequence Analysis, RNA
Single-Cell Analysis/methods
eng
60_published
true
peer-reviewed
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: epublish
University of Lausanne
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