serval:BIB_D9353C50A828
SPOT14-positive neural stem/progenitor cells in the hippocampus respond dynamically to neurogenic regulators.
10.1016/j.stemcr.2014.08.013
000345118600006
25418721
Knobloch
M.
author
von Schoultz
C.
author
Zurkirchen
L.
author
Braun
S.M.
author
Vidmar
M.
author
Jessberger
S.
author
article
2014-11-11
Stem cell reports
2213-6711
2213-6711
journal
3
5
735-742
Proliferation of neural stem/progenitor cells (NSPCs) in the adult brain is tightly controlled to prevent exhaustion and to ensure proper neurogenesis. Several extrinsic stimuli affect NSPC regulation. However, the lack of unique markers led to controversial results regarding the in vivo behavior of NSPCs to different stimuli. We recently identified SPOT14, which controls NSPC proliferation through regulation of de novo lipogenesis, selectively in low-proliferating NSPCs. Whether SPOT14-expressing (SPOT14+) NSPCs react in vivo to neurogenic regulators is not known. We show that aging is accompanied by a marked disappearance of SPOT14+ NSPCs, whereas running, a positive neurogenic stimulus, increases proliferation of SPOT14+ NSPCs. Furthermore, transient depletion of highly proliferative cells recruits SPOT14+ NSPCs into the proliferative pool. Additionally, we have established endogenous SPOT14 protein staining, reflecting transgenic SPOT14-GFP expression. Thus, our data identify SPOT14 as a potent marker for adult NSPCs that react dynamically to positive and negative neurogenic regulators.
Age Factors
Animals
Antineoplastic Agents, Alkylating/pharmacology
Biomarkers/metabolism
Cell Proliferation/drug effects
Dacarbazine/analogs & derivatives
Dacarbazine/pharmacology
Green Fluorescent Proteins/genetics
Green Fluorescent Proteins/metabolism
Hippocampus/cytology
Hippocampus/growth & development
Hippocampus/metabolism
Immunohistochemistry
Mice, Transgenic
Microscopy, Fluorescence
Neural Stem Cells/metabolism
Neurogenesis/drug effects
Nuclear Proteins/genetics
Nuclear Proteins/metabolism
Transcription Factors/genetics
Transcription Factors/metabolism
eng
60_published
true
peer-reviewed
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
University of Lausanne
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