serval:BIB_C4315A6F47A8
Development and evaluation of double locus sequence typing for molecular epidemiological investigations of Clostridium difficile.
10.1007/s10096-015-2525-4
000368813200003
26581425
Stojanov
M.
author
Magalhaes
B.
author
Terletsky
V.
author
Basset
P.
author
Prod'hom
G.
author
Greub
G.
author
Senn
L.
author
Blanc
D.S.
author
article
2016
European Journal of Clinical Microbiology and Infectious Diseases
1435-4373
0934-9723
journal
35
2
175-181
Despite the development of novel typing methods based on whole genome sequencing, most laboratories still rely on classical molecular methods for outbreak investigation or surveillance. Reference methods for Clostridium difficile include ribotyping and pulsed-field gel electrophoresis, which are band-comparing methods often difficult to establish and which require reference strain collections. Here, we present the double locus sequence typing (DLST) scheme as a tool to analyse C. difficile isolates. Using a collection of clinical C. difficile isolates recovered during a 1-year period, we evaluated the performance of DLST and compared the results to multilocus sequence typing (MLST), a sequence-based method that has been used to study the structure of bacterial populations and highlight major clones. DLST had a higher discriminatory power compared to MLST (Simpson's index of diversity of 0.979 versus 0.965) and successfully identified all isolates of the study (100 % typeability). Previous studies showed that the discriminatory power of ribotyping was comparable to that of MLST; thus, DLST might be more discriminatory than ribotyping. DLST is easy to establish and provides several advantages, including absence of DNA extraction [polymerase chain reaction (PCR) is performed on colonies], no specific instrumentation, low cost and unambiguous definition of types. Moreover, the implementation of a DLST typing scheme on an Internet database, such as that previously done for Staphylococcus aureus and Pseudomonas aeruginosa ( http://www.dlst.org ), will allow users to easily obtain the DLST type by submitting directly sequencing files and will avoid problems associated with multiple databases.
eng
60_published
true
peer-reviewed
Publication types: Journal ArticlePublication Status: ppublish
University of Lausanne
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