serval:BIB_BFECD1C3241A
A DNA barcode reference library for Swiss butterflies and forester moths as a tool for species identification, systematics and conservation.
10.1371/journal.pone.0208639
000454149400011
30576327
Litman
J.
author
Chittaro
Y.
author
Birrer
S.
author
Praz
C.
author
Wermeille
E.
author
Fluri
M.
author
Stalling
T.
author
Schmid
S.
author
Wyler
S.
author
Gonseth
Y.
author
article
2018
PloS one
1932-6203
1932-6203
journal
13
12
e0208639
Butterfly monitoring and Red List programs in Switzerland rely on a combination of observations and collection records to document changes in species distributions through time. While most butterflies can be identified using morphology, some taxa remain challenging, making it difficult to accurately map their distributions and develop appropriate conservation measures. In this paper, we explore the use of the DNA barcode (a fragment of the mitochondrial gene COI) as a tool for the identification of Swiss butterflies and forester moths (Rhopalocera and Zygaenidae). We present a national DNA barcode reference library including 868 sequences representing 217 out of 224 resident species, or 96.9% of Swiss fauna. DNA barcodes were diagnostic for nearly 90% of Swiss species. The remaining 10% represent cases of para- and polyphyly likely involving introgression or incomplete lineage sorting among closely related taxa. We demonstrate that integrative taxonomic methods incorporating a combination of morphological and genetic techniques result in a rate of species identification of over 96% in females and over 98% in males, higher than either morphology or DNA barcodes alone. We explore the use of the DNA barcode for exploring boundaries among taxa, understanding the geographical distribution of cryptic diversity and evaluating the status of purportedly endemic taxa. Finally, we discuss how DNA barcodes may be used to improve field practices and ultimately enhance conservation strategies.
Animals
Butterflies/classification
Butterflies/genetics
DNA/chemistry
DNA/isolation & purification
DNA/metabolism
DNA Barcoding, Taxonomic
Electron Transport Complex IV/classification
Electron Transport Complex IV/genetics
Female
Gene Library
Insect Proteins/classification
Insect Proteins/genetics
Male
Moths/classification
Moths/genetics
Switzerland
eng
60_published
true
peer-reviewed
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
University of Lausanne
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