serval:BIB_A9F33414833D
Mechanism of selective recruitment of RNA polymerases II and III to snRNA gene promoters.
10.1101/gad.314245.118
000436067400011
29785964
Dergai
O.
author
Cousin
P.
author
Gouge
J.
author
Satia
K.
author
Praz
V.
author
Kuhlman
T.
author
Lhôte
P.
author
Vannini
A.
author
Hernandez
N.
author
article
2018-05-01
Genes & development
1549-5477
0890-9369
journal
32
9-10
711-722
RNA polymerase II (Pol II) small nuclear RNA (snRNA) promoters and type 3 Pol III promoters have highly similar structures; both contain an interchangeable enhancer and "proximal sequence element" (PSE), which recruits the SNAP complex (SNAPc). The main distinguishing feature is the presence, in the type 3 promoters only, of a TATA box, which determines Pol III specificity. To understand the mechanism by which the absence or presence of a TATA box results in specific Pol recruitment, we examined how SNAPc and general transcription factors required for Pol II or Pol III transcription of SNAPc-dependent genes (i.e., TATA-box-binding protein [TBP], TFIIB, and TFIIA for Pol II transcription and TBP and BRF2 for Pol III transcription) assemble to ensure specific Pol recruitment. TFIIB and BRF2 could each, in a mutually exclusive fashion, be recruited to SNAPc. In contrast, TBP-TFIIB and TBP-BRF2 complexes were not recruited unless a TATA box was present, which allowed selective and efficient recruitment of the TBP-BRF2 complex. Thus, TBP both prevented BRF2 recruitment to Pol II promoters and enhanced BRF2 recruitment to Pol III promoters. On Pol II promoters, TBP recruitment was separate from TFIIB recruitment and enhanced by TFIIA. Our results provide a model for specific Pol recruitment at SNAPc-dependent promoters.
HEK293 Cells
Humans
Mutation
Promoter Regions, Genetic
Protein Binding
Protein Domains
Protein Transport
RNA Polymerase II/metabolism
RNA Polymerase III/metabolism
RNA, Small Nuclear/genetics
RNA, Small Nuclear/metabolism
TATA Box/genetics
TATA-Box Binding Protein/metabolism
Transcription Factor TFIIB/metabolism
Transcription Factors/metabolism
BRF2
SNAPc
TBP
TFIIA
TFIIB
small nuclear RNA promoters
eng
60_published
true
peer-reviewed
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
University of Lausanne
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