serval:BIB_A662BC1558A2
3D reconstruction and comparison of shapes of DNA minicircles observed by cryo-electron microscopy.
10.1093/nar/gkl675
000241955100008
17012274
Amzallag
A.
author
Vaillant
C.
author
Jacob
M.
author
Unser
M.
author
Bednar
J.
author
Kahn
J.D.
author
Dubochet
J.
author
Stasiak
A.
author
Maddocks
J.H.
author
article
2006
Nucleic Acids Research
1362-4962[electronic], 0305-1048[linking]
journal
34
18
e125
We use cryo-electron microscopy to compare 3D shapes of 158 bp long DNA minicircles that differ only in the sequence within an 18 bp block containing either a TATA box or a catabolite activator protein binding site. We present a sorting algorithm that correlates the reconstructed shapes and groups them into distinct categories. We conclude that the presence of the TATA box sequence, which is believed to be easily bent, does not significantly affect the observed shapes.
Algorithms
Base Sequence
Binding Sites
Cluster Analysis
Cryoelectron Microscopy
Cyclic AMP Receptor Protein/metabolism
DNA, Circular/chemistry
DNA, Circular/metabolism
Imaging, Three-Dimensional
Molecular Sequence Data
Motion
Nucleic Acid Conformation
Software
TATA Box
eng
60_published
true
peer-reviewed
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
University of Lausanne
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