serval:BIB_969A35C25E60
Comparative gene finding in chicken indicates that we are closing in on the set of multi-exonic widely expressed human genes.
10.1093/nar/gki328
000228398400031
15809229
Castelo
R.
author
Reymond
A.
author
Wyss
C.
author
Câmara
F.
author
Parra
G.
author
Antonarakis
S.E.
author
Guigó
R.
author
Eyras
E.
author
article
2005
Nucleic Acids Research
1362-4962[electronic], 0305-1048[linking]
journal
33
6
1935-1939
The recent availability of the chicken genome sequence poses the question of whether there are human protein-coding genes conserved in chicken that are currently not included in the human gene catalog. Here, we show, using comparative gene finding followed by experimental verification of exon pairs by RT-PCR, that the addition to the multi-exonic subset of this catalog could be as little as 0.2%, suggesting that we may be closing in on the human gene set. Our protocol, however, has two shortcomings: (i) the bioinformatic screening of the predicted genes, applied to filter out false positives, cannot handle intronless genes; and (ii) the experimental verification could fail to identify expression at a specific developmental time. This highlights the importance of developing methods that could provide a reliable estimate of the number of these two types of genes.
Animals
Chickens/genetics
Computational Biology/methods
Exons
Gene Expression
Genome, Human
Genomics/methods
Humans
Reverse Transcriptase Polymerase Chain Reaction
eng
60_published
true
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
University of Lausanne
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