serval:BIB_8BF899F5DFF5
Endogenous glutathione levels modulate the frequency of both spontaneous and long wavelength ultraviolet induced mutations in human cells
10.1093/carcin/13.9.1557
A1992JP54700011
1394839
Applegate
L. A.
author
Lautier
D.
author
Frenk
E.
author
Tyrrell
R. M.
author
article
1992
Carcinogenesis
0143-3334
journal
13
9
1557-1560
Spontaneous and induced mutations at the hypoxanthine guanine phosphoribosyl transferase locus have been measured in cultured human lymphoblastoid (TK6) cell populations under conditions in which cellular glutathione has been severely depleted by overnight treatment with buthionine-S,R-sulfoximine. At maximum levels of glutathione depletion, the increase in spontaneous frequency is at least 5-fold, a finding consistent with the possibility that cellular redox state can modulate the levels of pre-mutagenic damage arising as a result of normal metabolism in cultured human cells. Glutathione depletion does not lead to a significant enhancement in the frequency of mutants that arise as a result of irradiation at 313 nm but does lead to a 3-fold increase in mutations resulting from irradiation at 365 nm. These results indicate that glutathione may quench reactive intermediates that would otherwise lead to spontaneous mutations as well as a fraction of UVA radiation-induced premutagenic damage
analogs & derivatives/Buthionine Sulfoximine/Cells/Cells,Cultured/deficiency/genetics/Glutathione/Humans/Hypoxanthine Phosphoribosyltransferase/metabolism/Methionine Sulfoximine/Mutation/pharmacology/physiology/Radiation-Sensitizing Agents/Ultraviolet Rays
60_published
true
DA - 19921029
LA - eng
PT - Journal Article
PT - Research Support, Non-U.S. Gov't
RN - 0 (Radiation-Sensitizing Agents)
RN - 1982-67-8 (Methionine Sulfoximine)
RN - 5072-26-4 (Buthionine Sulfoximine)
RN - 70-18-8 (Glutathione)
RN - EC 2.4.2.8 (Hypoxanthine Phosphoribosyltransferase)
SB - IM
University of Lausanne
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