serval:BIB_7A89BB65CC85
Heterotrimeric Go protein links Wnt-Frizzled signaling with ankyrins to regulate the neuronal microtubule cytoskeleton.
10.1242/dev.106773
25139856
000341305900014
Lüchtenborg
A.M.
author
Solis
G.P.
author
Egger-Adam
D.
author
Koval
A.
author
Lin
C.
author
Blanchard
M.G.
author
Kellenberger
S.
author
Katanaev
V.L.
author
article
2014
Development
1477-9129
0950-1991
journal
141
17
3399-3409
Drosophila neuromuscular junctions (NMJs) represent a powerful model system with which to study glutamatergic synapse formation and remodeling. Several proteins have been implicated in these processes, including components of canonical Wingless (Drosophila Wnt1) signaling and the giant isoforms of the membrane-cytoskeleton linker Ankyrin 2, but possible interconnections and cooperation between these proteins were unknown. Here, we demonstrate that the heterotrimeric G protein Go functions as a transducer of Wingless-Frizzled 2 signaling in the synapse. We identify Ankyrin 2 as a target of Go signaling required for NMJ formation. Moreover, the Go-ankyrin interaction is conserved in the mammalian neurite outgrowth pathway. Without ankyrins, a major switch in the Go-induced neuronal cytoskeleton program is observed, from microtubule-dependent neurite outgrowth to actin-dependent lamellopodial induction. These findings describe a novel mechanism regulating the microtubule cytoskeleton in the nervous system. Our work in Drosophila and mammalian cells suggests that this mechanism might be generally applicable in nervous system development and function.
eng
60_published
peer-reviewed
Publication types: Journal Article Publication Status: ppublish
University of Lausanne
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