serval:BIB_724D1DA74EF4
Decreased activity of inducible nitric oxide synthase type 2 and modulation of the expression of glutathione S-transferase alpha, bcl-2, and metallothioneins during the differentiation of CaCo-2 cells.
9040948
A1997WG49100015
Vecchini
F.
author
Pringault
E.
author
Billiar
T.R.
author
Geller
D.A.
author
Hausel
P.
author
Felley-Bosco
E.
author
article
1997
Cell Growth and Differentiation
1044-9523
1044-9523
journal
8
2
261-268
Reactive oxygen species modulate the cell growth of a wide variety of mammalian cells. To determine whether oxidative metabolism is altered during the differentiation process, we studied the expression of pro- and antioxidant proteins in proliferating and differentiated CaCo-2 cells, a human colon adenocarcinoma cell line. Nitric oxide synthase type 2 (iNOS) produces nitric oxide (NO). Depending on its rate of synthesis, NO may either promote cellular and DNA damage or reduce the ability of other free radicals to induce cell injury. Using Western and Northern blot analysis and arginine conversion assay, we demonstrate that the expression of iNOS decreases when cells undergo differentiation. This biological event entails a diminished production of NO metabolites and correlates with the loss of activation of soluble guanylate cyclase activity. In differentiated cells, a 2-fold down-regulation of the nuclear factor kappa B activity was observed, suggesting that nuclear factor kappa B could be one of the iNOS gene regulatory factors in the CaCo-2 model. In parallel, we studied the expression of other antioxidant proteins including glutathione S-transferase alpha (GST alpha), bcl-2, and the metallothioneins (MTs). We show that the protein levels of GST alpha and MT increase during the differentiation of CaCo-2 cells, whereas bcl-2 levels decrease. Our investigation indicates that the expression of iNOS, GST alpha, bcl-2, and MT is associated with the enterocytic differentiation. The shift in the expression of specific antioxidant genes during CaCo-2 cell differentiation may occur to avoid alterations in the cell redox potential.
Actins/chemistry
Blotting, Western
Caco-2 Cells
Cell Differentiation/genetics
Cell Differentiation/physiology
Cyclic GMP/chemistry
Enzyme Induction
Gene Expression Regulation/physiology
Glutathione Transferase/biosynthesis
Glutathione Transferase/genetics
Humans
Intestines/cytology
Metallothionein/biosynthesis
Metallothionein/genetics
Models, Biological
Nitric Oxide Synthase/biosynthesis
Nitric Oxide Synthase/genetics
Proto-Oncogene Proteins c-bcl-2/biosynthesis
Tumor Suppressor Protein p53/chemistry
eng
60_published
peer-reviewed
Publication types: Journal Article
Publication Status: ppublish
University of Lausanne
mailto:serval_help@unil.ch
http://www.unil.ch/serval
http://serval.unil.ch/disclaimer
https://serval.unil.ch/notice/serval:BIB_724D1DA74EF4