serval:BIB_643E20D79827
Clock-dependent chromatin topology modulates circadian transcription and behavior.
10.1101/gad.312397.118
000428519800005
29572261
Mermet
J.
author
Yeung
J.
author
Hurni
C.
author
Mauvoisin
D.
author
Gustafson
K.
author
Jouffe
C.
author
Nicolas
D.
author
Emmenegger
Y.
author
Gobet
C.
author
Franken
P.
author
Gachon
F.
author
Naef
F.
author
article
2018-03-01
Genes & development
1549-5477
0890-9369
journal
32
5-6
347-358
The circadian clock in animals orchestrates widespread oscillatory gene expression programs, which underlie 24-h rhythms in behavior and physiology. Several studies have shown the possible roles of transcription factors and chromatin marks in controlling cyclic gene expression. However, how daily active enhancers modulate rhythmic gene transcription in mammalian tissues is not known. Using circular chromosome conformation capture (4C) combined with sequencing (4C-seq), we discovered oscillatory promoter-enhancer interactions along the 24-h cycle in the mouse liver and kidney. Rhythms in chromatin interactions were abolished in arrhythmic <i>Bmal1</i> knockout mice. Deleting a contacted intronic enhancer element in the <i>Cryptochrome 1</i> ( <i>Cry1</i> ) gene was sufficient to compromise the rhythmic chromatin contacts in tissues. Moreover, the deletion reduced the daily dynamics of <i>Cry1</i> transcriptional burst frequency and, remarkably, shortened the circadian period of locomotor activity rhythms. Our results establish oscillating and clock-controlled promoter-enhancer looping as a regulatory layer underlying circadian transcription and behavior.
Animals
CLOCK Proteins/genetics
Chromatin/genetics
Chromatin/metabolism
Circadian Rhythm/genetics
Cryptochromes/genetics
Cryptochromes/metabolism
Enhancer Elements, Genetic/genetics
Kidney/physiology
Liver/physiology
Mice
Mice, Knockout
Promoter Regions, Genetic/physiology
Sequence Deletion/genetics
Transcription, Genetic/genetics
DNA regulatory elements
chromatin topology
circadian rhythms
promoter–enhancer loops
transcriptional bursting
eng
60_published
true
peer-reviewed
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
University of Lausanne
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