serval:BIB_6214CD4B88BA
Coat color dilution in mice because of inactivation of the melanoma antigen MART-1.
10.1111/j.1755-148X.2011.00910.x
21943097
000298577600008
Aydin
I.T.
author
Hummler
E.
author
Smit
N.P.
author
Beermann
F.
author
article
2012-10
Pigment Cell and Melanoma Research
1755-148X
1755-1471
journal
25
1
37-46
Melanoma antigen recognized by T cells 1 (MART-1) is a melanoma-specific antigen, which has been thoroughly studied in the context of immunotherapy against malignant melanoma and which is found only in the pigment cell lineage. However, its exact function and involvement in pigmentation is not clearly understood. Melanoma antigen recognized by T cells 1 has been shown to interact with the melanosomal proteins Pmel17 and OA1. To understand the function of MART-1 in pigmentation, we developed a new knockout mouse model. Mice deficient in MART-1 are viable, but loss of MART-1 leads to a coat color phenotype, with a reduction in total melanin content of the skin and hair. Lack of MART-1 did not affect localization of melanocyte-specific proteins nor maturation of Pmel17. Melanosomes of hair follicle melanocytes in MART-1 knockout mice displayed morphological abnormalities, which were exclusive to stage III and IV melanosomes. In conclusion, our results suggest that MART-1 is a pigmentation gene that is required for melanosome biogenesis and/or maintenance.
Animals
Cells, Cultured/metabolism
Genetic Vectors
Hair/metabolism
Hair Color/genetics
MART-1 Antigen/genetics
MART-1 Antigen/physiology
Melanins/metabolism
Melanocytes/metabolism
Melanosomes/metabolism
Melanosomes/pathology
Mice
Mice, Inbred C57BL
Mice, Knockout
Phenotype
Skin/cytology
Skin/metabolism
gp100 Melanoma Antigen/metabolism
eng
60_published
peer-reviewed
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
University of Lausanne
mailto:serval_help@unil.ch
http://www.unil.ch/serval
http://serval.unil.ch/disclaimer
https://serval.unil.ch/notice/serval:BIB_6214CD4B88BA