serval:BIB_2B9CC70589A8
Microfluidics for rapid cytokeratin immunohistochemical staining in frozen sections.
10.1038/labinvest.2017.49
000406399500009
28553936
Brajkovic
S.
author
Dupouy
D.G.
author
de Leval
L.
author
Gijs
M.A.
author
article
2017-08
Laboratory investigation; a journal of technical methods and pathology
1530-0307
0023-6837
journal
97
8
983-991
Frozen sections (FS) of tumor samples represent a cornerstone of pathological intraoperative consultation and have an important role in the microscopic analysis of specimens during surgery. So far, immunohistochemical (IHC) stainings on FS have been demonstrated for a few markers using manual methods. Microfluidic technologies have proven to bring substantial improvement in many fields of diagnostics, though only a few microfluidic devices have been designed to improve the performance of IHC assays. In this work, we show optimization of a complete pan-cytokeratin chromogenic immunostaining protocol on FS using a microfluidic tissue processor into a protocol taking <12 min. Our results showed specificity and low levels of background. The dimensions of the microfluidic prototype device are compatible with the space constraints of an intraoperative pathology laboratory. We therefore anticipate that the adoption of microfluidic technologies in the field of surgical pathology can significantly improve the way FSs influence surgical procedures.
Breast/diagnostic imaging
Coloring Agents/chemistry
Equipment Design
Female
Humans
Immunohistochemistry/instrumentation
Immunohistochemistry/methods
Keratins/analysis
Keratins/chemistry
Keratins/metabolism
Male
Microfluidic Analytical Techniques/instrumentation
Microfluidic Analytical Techniques/methods
Neoplasms/diagnostic imaging
Prostate/diagnostic imaging
Ureter/diagnostic imaging
eng
60_published
true
peer-reviewed
Publication types: Journal Article
Publication Status: ppublish
University of Lausanne
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