serval:BIB_2A9ED0B306D3
Vasoactive intestinal peptide, pituitary adenylate cyclase-activating peptide, and noradrenaline induce the transcription factors CCAAT/enhancer binding protein (C/EBP)-beta and C/EBP delta in mouse cortical astrocytes: involvement in cAMP-regulated glycogen metabolism.
8558260
A1996TQ57300006
Cardinaux
J.R.
author
Magistretti
P.J.
author
article
1996
Journal of Neuroscience
0270-6474
journal
16
3
919-929
We have described previously a transcription-dependent induction of glycogen resynthesis by the vasoactive intestinal peptide (VIP) or noradrenaline (NA) in astrocytes, which is mediated by cAMP. Because it has been postulated that the cAMP-mediated regulation of energy balance in hepatocytes and adipocytes is channeled at least in part through the CCAAT/enhancer binding protein (C/EBP) family of transcription factors, we tested the hypothesis that C/EBP isoforms could be expressed in mouse cortical astrocytes and that their level of expression could be regulated by VIP, by the VIP-related neuropeptide pituitary adenylate cyclase-activating peptide (PACAP), or by NA. We report in this study that in these cells, C/EBP beta and C/EBP delta are induced by VIP, PACAP, or NA via the cAMP second-messenger pathway. Induction of C/EBP beta and -delta mRNA by VIP occurs in the presence of a protein synthesis inhibitor. Thus, c/ebp beta and c/ebp delta behave as cAMP-inducible immediate-early genes in astrocytes. Moreover, transfection of astrocytes with expression vectors selectively producing the transcriptionally active form of C/EBP beta, termed liver-enriched transcriptional activator protein, or C/EBP delta enhance the glycogen resynthesis elicited by NA, whereas an expression vector producing the transcriptionally inactive form of C/EBP beta, termed liver-enriched transcriptional inhibitory protein, reduces this resynthesis. These results support the idea that C/EBP beta and -delta regulate gene expression of energy metabolism-related enzymes in astrocytes.
8-Bromo Cyclic Adenosine Monophosphate
Animals
Anisomycin
Astrocytes
CCAAT-Enhancer-Binding Protein-delta
CCAAT-Enhancer-Binding Proteins
Cells, Cultured
Cerebral Cortex
Cyclic AMP
DNA-Binding Proteins
Energy Metabolism
Forskolin
Gene Expression Regulation
Genes, Immediate-Early
Glycogen
Leucine Zippers
Mice
Nerve Tissue Proteins
Neuropeptides
Norepinephrine
Nuclear Proteins
Pituitary Adenylate Cyclase-Activating Polypeptide
Protein Synthesis Inhibitors
Second Messenger Systems
Transcription Factors
Transcription, Genetic
Vasoactive Intestinal Peptide
eng
60_published
peer-reviewed
University of Lausanne
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